SVIBOR - Project code: 4-07-061


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Project code: 4-07-061


Main researcher: ŠTRUCELJ, DUBRAVKA (48402)

Type of research: applied
Duration from: 01/01/91. to 02/28/95.

Papers on project (total): 31
Papers on project quoted in Current Contents: 3
Institution name: Prehrambeno-biotehnološki fakultet, Zagreb (58)
Department/Institute: Department of Food Technology
Address: Pierottijeva 6
City: 10000 - Zagreb, Croatia
Phone: 385 (0)41 440-089
Fax: 385 (0)41 418-230

Summary: The effect of soybean pretreatment on the phospholipid content and composition in crude and degummed oils was investigated and commercial (industrial, technical) lecithin was bleached with 1% of hydrogene peroxide. The developed procedures of alcohol fractionation of commercial lecithin were carried out in two stages on laboratory- and pilot - scale. By means of ethanol fractionation in a 12oo mm glass column ( 86 mm i.d.) several phosphatidylcholine (PC) - enriched fractions with improved PC/PE ratio were obtained: the Extract I. with 56.15% PC and PC/PE ratio 4.01 or two alcohol fractions with 38.49% / 67.03% PC and PC/PE ratio 1.98/6.26. The conditions of laboratory continous counter current extraction of lecithin with hexane/acetone mixtures were studdied in order to obtain good yield (46.0 %) of powdered lecithin with low triacylglycerol content ( 96.1 % acetone insoluble). During the experiments performed,the changes of phospholipid composition were determined by TLC and certain lecithin fractions were analysed by HPLC. Emulsifying and antioxidative properties of commercial lecithin, fractions and individual phospholipids were investigated in laboratory - scale. Commercial lecithin was the best emulsifying agens in ordinary o/w emulsions and PC in submicron ones. Results of lard oxidative stability determination indicate the best inhibition activity of commercial lecithin.

Keywords: Acetone, alcohol, antioxidative activity, bleached lecithin, degumming, emulsions, emulsifying activity, extraction, ethanol, fractionation, HPLC, lard, lecithin, phospholipids, phosphatidylcholine, oxidative stability, powdered lecithin, soybean oil, TLC.

Research goals: Project proposal, registered in the category of developing investigations, was based on our previous papers and experience from the collaboration with Soybean Processing Plant in Zadar, the greatest producer of industrial lecithin. At that time the financial support was expected for the study of the improvement of the existing technology of soybean oil and lecithin as well as for the development of new procedures of further lecithin processing. But, still before the war escalated and the supposed collaboration became impossible, it was evident that the complex problem of lecithin fractionation could not be investigated without the considerable incorporation of theoretical presuppositions. Therefore, the reults of this project, undoubtely, contribute to the progress of the science and technology so it could be suggested to transfer it to the category of a p p l i e d scientific investigations. Alcohol fractionation of soybean lecithin was carried out with a purpose to obtain several phospholipid fractions, among which the fractions with increased content of phosphatidylcholine (PC) and PC/PE ratio over 4 are the most suitable for application as the emulsifiers in food products and for including into the pharmaceutical preparations. A powdered lecithin, obtained through acetone fractionation of lecithin has a wide application in food products for special purposes as well as in pharmaceutical products.


  1. Name of institution: Faculty of Agricultural Sciences
    Type of institution: University/Faculty
    Type of cooperation: Joint publishing of scientific papers
    City: Gent, Belgija

  2. Name of institution: Agrokor-Tvornica za preradu soje
    Type of institution: Economical/Production
    Type of cooperation: Occasional exchange of information
    City: 23000 - Zadar, Croatia

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Last update: 10/10/95