DEVELOPMENT OF SOYBEAN LECITHIN MANUFACTURE AND FRACTIONATION
Main researcher
: ŠTRUCELJ, DUBRAVKA (48402) Assistants
JERIČEVIĆ, DUŠAN (144985)
RADE, DESANKA (39551)
JOVANOVIĆ, KARMELA (18915)
DOMINEZ, JELENA (10273)
MOKROVČAK, ŽELJKO (32312)
KONEČNY, DUBRAVKA (900713)
Type of research: applied Duration from: 01/01/91. to 02/28/95. Papers on project (total): 31
Papers on project quoted in Current Contents: 3
Institution name: Prehrambeno-biotehnološki fakultet, Zagreb (58) Department/Institute: Department of Food Technology Address: Pierottijeva 6 City: 10000 - Zagreb, Croatia
Communication
Phone: 385 (0)41 440-089
Fax: 385 (0)41 418-230
Summary: The effect of soybean pretreatment on the phospholipid
content and composition in crude and degummed oils was investigated and
commercial (industrial, technical) lecithin was bleached with 1% of
hydrogene peroxide. The developed procedures of alcohol
fractionation of commercial lecithin were carried out in two stages on
laboratory- and pilot - scale. By means of ethanol fractionation in a 12oo
mm glass column ( 86 mm i.d.) several phosphatidylcholine (PC) - enriched
fractions with improved PC/PE ratio were obtained: the Extract I. with
56.15% PC and PC/PE ratio 4.01 or two alcohol fractions with 38.49% /
67.03% PC and PC/PE ratio 1.98/6.26. The conditions of laboratory continous
counter current extraction of lecithin with hexane/acetone mixtures were
studdied in order to obtain good yield (46.0 %) of powdered lecithin with
low triacylglycerol content ( 96.1 % acetone insoluble). During the
experiments performed,the changes of phospholipid composition were
determined by TLC and certain lecithin fractions were analysed by HPLC.
Emulsifying and antioxidative properties of commercial lecithin, fractions
and individual phospholipids were investigated in laboratory - scale.
Commercial lecithin was the best emulsifying agens in ordinary o/w
emulsions and PC in submicron ones. Results of lard oxidative stability
determination indicate the best inhibition activity of commercial lecithin.
Research goals: Project proposal, registered in the category of
developing investigations, was based on our previous papers and experience
from the collaboration with Soybean Processing Plant in Zadar, the greatest
producer of industrial lecithin. At that time the financial support was
expected for the study of the improvement of the existing technology of
soybean oil and lecithin as well as for the development of new procedures
of further lecithin processing. But, still before the war escalated and the
supposed collaboration became impossible, it was evident that the complex
problem of lecithin fractionation could not be investigated without the
considerable incorporation of theoretical presuppositions. Therefore, the
reults of this project, undoubtely, contribute to the progress of the
science and technology so it could be suggested to transfer it to the
category of a p p l i e d scientific investigations. Alcohol
fractionation of soybean lecithin was carried out with a purpose to obtain
several phospholipid fractions, among which the fractions with increased
content of phosphatidylcholine (PC) and PC/PE ratio over 4 are the most
suitable for application as the emulsifiers in food products and for
including into the pharmaceutical preparations. A powdered lecithin,
obtained through acetone fractionation of lecithin has a wide application
in food products for special purposes as well as in pharmaceutical
products.
COOPERATION - INSTITUTIONS
Name of institution
: Faculty of Agricultural Sciences Type of institution: University/Faculty Type of cooperation: Joint publishing of scientific papers City: Gent, Belgija
Name of institution
: Agrokor-Tvornica za preradu soje Type of institution: Economical/Production Type of cooperation: Occasional exchange of information City: 23000 - Zadar, Croatia Other information about the project.