SVIBOR - Project code: 3-01-041

MINISTRY OF SCIENCE AND TECHNOLOGY

Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69
E-mail: ured@znanost.hr

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Project code: 3-01-041

INFLUENCE OF ORCHIDECTOMY AND STEROID HORMONES ON VARIOUS ORGANS

Main researcher: BANEK, LJERKA (76706)

Assistants

PEZEROVIĆ, RUŽICA (34856)
JEŽEK, DAVOR (171511)

Type of research: applied
Duration from: 01/01/91. to 12/15/96.

Papers on project (total): 54
Papers on project quoted in Current Contents: 8

Institution name: Medicinski fakultet, Zagreb (108)
Department/Institute: Institute of Histology and Embryology, Medical Faculty University of Zagreb
Address: Šalata 3
City: 10000 - Zagreb, Croatia

Communication: Phone: 385 (0) 1 456 67 93; Phone: 385 (0)1 456 69 64; Fax: 385 (0)1 42 40 01; E-mail: davor@mamef.mef.hr

Summary: This study deals with morphological changes caused by a testis removal (orchidectomy) on parotid and parathyroid gland (as well es cytological and electron microscopical study of healtly parathyroid glands). Also,influence of high and low doses of testosterone in blood (caused by exogenously given testosterone and in cryptorchidism) on testis and spermatogenesis is studied. Part of investigation is dedicated to an action of steroid hormones on rat genital ridge development. Methods of the study comprise several experiments on rats. After the experiment is completed, the structure of theabove mentioned organs is qualitatively and quantitatively analysed by histological methods (light and electron microscopy). Results of investigation for the first time describe the existence of light and dark cells in the acini of the rat parotid gland. After orchidectomy, volume of these cells is changed aswell as the length of parotid duct system. The above mentioned effects of orchidectomy can be prevented by exogenously giventestosterone. Furthermore, for the first time a comparative study dealing with negative effects of high doses of testosterone enanthate and testosterone propionate has been described. Methods used in the study were light and electron microscopy. A relationship between light and dark Sertoli cells in cryptorchid human testis has been investigated, as well as differentiation of genital ridge in various experimental conditions. Peritubular (myoid) cells of fetal human testes have been investigated by immunohistochemical and stereological methods. This is the first report on an early development of human myoid cells. Macrophages in the testis play an important role in the paracrine regulation of Leydig cells. In the rats treated with high doses of testosterone we have analyzed testicular macrophages by immunohistochemical and electron microscopy methods. After the testosterone treatment, these cells seem to be activated. In human infertile testes we have noticed a decresed length and curvature of seminiferous tubules as well as changed architecture of blood vessels. These vesseles as well as the lamina propria of seminiferous tubules were analyzed by immunohistochemical and electron microscopy methods. Peritubular cells of the lamina propria showed a disorganisation of desmin, and an enlargement of collagen network.

Keywords: TESTIS, ORCHIDECTOMY, PAROTID GLAND, PARATHYROID GLAND, TESTOSTERONE, GONADAL DEVELOPMENT, GENITAL RIDGE, CRYPTORCHIDISM, SERTOLI CELLS, MYOID CELLS, TESTICULAR MACROPHGAES

Research goals: Since parotid gland binds bigger quantity of testosterone from the blood than prostate, it can be assumed that this hormone participate in the parotid gland structure and function. It has been established that ovariectomy causes a decrease in the parathyroid gland function. However, the influence of orchidectomy on this gland has not been studied so far. Testosterone is essential for normal spermatogenesis. Since high doses of this hormone are tested for contraceptive activity in males, it is necessary to establish how high level of testosterone influence various testis parameters (the length and curvature of seminiferous tubules, Sertoli, Leydig,myoid cells and testicular macrophages). Cryptorchid testes produce significantly lower levels of endogenous testosterone. Therefore, it is presumed that, as a consequence of a such testosterone production, a morphological changes of cells in cytological smears of human testes could occur. Identification of these changes is important for a diagnosis and determination of a degree of the testis damage. Knowledge about the influence of steroid hormones on the genital ridge development could explain some anomalies in the development of the genital tract. On the basis of all mentioned above, aims of this project are as follows: a) studying of effects of orchidectomy on the structure and function of the parotid and parathyroid gland b) assessment of the effects of high and low doses of testosterone in blood on the testis structure c) determination of the role of steroid hormones on the genital ridge differentiation.

COOPERATION - INSTITUTIONS

Name of institution: Zavod za farmakologiju Medicinskog fakulteta Sveučlišta u Zagrebu, Šalata
Type of institution: University/Faculty
Type of cooperation: Joint publishing of scientific papers
City: 10000 - Zagreb, Croatia
Name of institution: Patologische Institut Universitat Innsbruck
Type of institution: University/Faculty
Type of cooperation: Joint publishing of scientific papers
City: Innsbruck, AUSTRIJA
Name of institution: Universitat Krankenhaus Eppendorf, Abteilung fur Andrologie
Type of institution: University/Faculty
Type of cooperation: Joint publishing of scientific papers
City: Hamburg, Njemačka
Name of institution: Klinička bolnica Dubrava, Odjel za nuklearnu medicinu
Type of institution: University/Faculty
Type of cooperation: Joint project
City: 10000 - Zagreb, Croatia
Name of institution: -

Other information about the project.


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