Organic Solvents - Biological Monitoring and Toxic Effects
Main researcher
: MAJIĆ-PRPIĆ, DANICA (27410) Assistants
BOGADI-ŠARE, ANA (113110)
KARAČIĆ, VIŠNJA (20151)
PIŠL, ZORAN (82896)
SKENDER, LJILJANA (43516)
UGRENOVIĆ, ŽELJKO (64025)
TURK, RAJKA (54592)
Type of research: basic Duration from: 01/01/91. to 12/31/94. Papers on project (total): 40
Papers on project quoted in Current Contents: 6
Institution name: Institut za medicinska istraživanja i medicinu rada, Zagreb (22) Department/Institute: Clinical-Toxicological Laboratory Address: Ksaverska cesta 2, P.P. 291 City: 10000 - Zagreb, Croatia
Communication
Phone: 385 (0)10 221573
Phone: 385 (0)10 214460
Fax: 385 (0)10 274572
Fax: 385 (0)10 221252
E-mail: ferencak@mimi.imi.hr
Summary: Within this project a study was conducted of human exposure
to trichloroethylene and tetrachloroethylene which are frequent
contaminants in the urban environment and a study of toluene
neurotoxicity in humans and benzene haematotoxicity in humans and in
experimental animals. The results of the study constitute a contribution
to science through the following conclusions: trichloroethylene and
tetrachloroethylene absorption in the general population under normal
environmental conditions was confirmatively established with levels
similar to those referred in Germany; in low toluene exposure, in spite
of the general neurologic findings in normal range, the central
nervous system impairment was proved measuring visual, cognitive and
brain stem evoked potentials and through determination of the
prevalence of acquired colour vision loss; in low benzene exposure, in
addition to complete blood cell count including red cell indices,
increased resistance to the hemolytical action of glycerol was found
to be the appropriate test for detecting early benzene haematotoxicity
and benzene in blood the appropriate biomarker of low-level benzene
exposure. A cytogenetic study showed the prevalence of chromosome-type
abberations (acentric fragments) and higher sister chromatid
exchanges frequency in benzene exposed subjects. Through an
experimental study it was demonstrated that the test for granulocyte
reserves is useful in assessing haematotoxic effects in benzene
poisoning. The results of the project have been published in 15
publications in scientific journals, 2 congress proceedings, 18
congress communications, 3 disertations and 1 master work.
Keywords: organic solvents, biological monitoring, trichloroethylene, tetrachloroethylene, neurotoxicity, central nervous system impairment, electrocortical activity, evoked brain potentials, colour vision loss, haematotoxic effects, benzene, glycerol haemolysis time, bone marrow granulocyte reserve
Research goals: In this project the successful implementation of
biological monitoring of exposure, the development of validated sensitive
and specific neurologic tests identifying early signs of solvent
neurotoxicity and the discovery of early indicators of solvent
haematotoxicity were interrelated in three objectives. The first
objective was to assess exposure of the general population to
trichloroethylene and tetrachloroethylene under normal environmental
conditions through biological monitoring. The second objective was to
investigate central nervous system impairment in long term exposure to low
levels of toluene verified by biological monitoring, by measurement of
evoked brain potentials and a general neurologic examination. A test for
the acquired colour vision loss (dyschromatopsia) as an indicator of
solvent neural alterations was also planned. The third objective was
related to the study of early indicators of benzene toxic effect on the
haematopoietic system in low levels of exposure. An additional study was
experimental, in which granulocyte response to glucocorticoid stimulation
in chronic benzene poisoning was examined. Other information about the project.