SVIBOR - Project code: 3-01-516

MINISTRY OF SCIENCE AND TECHNOLOGY

Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69
E-mail: ured@znanost.hr

SVIBOR

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Project code: 3-01-516


Molecular relationship between bone and bone marrow


Main researcher: MARUŠIĆ, ANA (136152)


Assistants
Type of research: basic
Duration from: 09/01/93. to 09/01/96.
Papers on project (total): 7
Papers on project quoted in Current Contents: 4
Institution name: Medicinski fakultet, Zagreb (108)
Department/Institute: Department of Anatomy
Address: Šalata 11, p.p. 916
City: 10000 - Zagreb, Croatia
Communication
Phone: 385 (0)1 45 66 825
Fax: 385 (0)1 27 11 95
E-mail: marusica@mamef.mef.hr
Summary: The relationship between bone and bone marrow is constant and manyfold, and disturbances in one system lead to disturbances in the other. The understanding of the nature of the relationship between bone and bone marrow on cellular and molecular level is the main aim of the proposed research. Stromal cells from the human bone marrow will be grown in vitro in conditions promoting their differentiation towards osteoblastic phenotype. The expression of osteoblast-specific genes will be determned using biochemical and gene markers. Special attention will be given to the osteogenic potential of bone marrow in different hematological disturbances (myelodysplastic syndrome, aplastic anemia etc.). In vivo, the role of the immunological system will be investigated in the mouse models of different immunodeficient states. Bone differentiation will be studied in the model of osteoinduction by demineralized bone matrix or bone marrow and will be evaluated histologically, biochemically and genetically.

Keywords: bone, bone marrow, osteoblast, stromal cells, differentiation, immunodefficiencies, immune system

Research goals: The main aim of the proposed research is the study of the interrelationship between bone and bone marrow on cellular and molecular level. Although the osteogenic potential of the bone marrow has been described, there is little knowledge on the sequence of events and factors influencing the osteogenic differentiation in bone marrow. The first direct aim of the proposed research is the determination of genetic differentiation markers of stromal system in bone marrow. This is especially relevant to the bone changes in hematological disorders, which have been sporadically and inconsistently described in the literature. In vivo work on mouse models of immunodeficient states, where it is possible to generate subtle changes in different cell linegaes of the hematopoetic system, will facilitate the investigation of the role of different parts of the immune system in osteogenesis. The expected contribution of the proposed research is in the unification of the research on bone and bone marrow. Up to the present, the research on those two anatomically close and related tissues has been scarce, especially in vivo. The elucidation of the differentiation sequence of the bone marrow stromal system would improve the understanding of the relationships between bone and bone marrow. The understanding of the balance between bone and bone marrow in health and disease would also improve the treatment and prognosis of the bone changes and recovery in hematologic disorders.

COOPERATION - PROJECTS


  1. Name of project: Identification of molecular markers of human bone cells: their use in defining interactions between bone cells and bone-marrow
    Name of institution: Institut fuer Pathologie, GSF Muenchen, SRNj - u okviru bilateralne znanstvene i tehničke suradnje SRNj i RH - Forschungszentrum Juelich
    City: Juelich, SR Njemačka

  2. Name of project: JF199 The role of the immune system in new bone formation
    Name of institution: National Institutes for Health
    City: Bethesda, S.A.D.
Other information about the project.
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