SVIBOR - Project code: 3-03-294


Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69


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Project code: 3-03-294


Main researcher: KRALJEVIĆ, PETAR (23266)

Type of research: basic
Duration from: 01/01/91. to 12/15/97.

Papers on project (total): 19
Institution name: Veterinarski fakultet, Zagreb (53)
Department/Institute: Department for Physiology and Radiobiology
Address: Heinzelova 55
City: 10000 - Zagreb, Croatia
Phone: 385 (0)41 290 178

Summary: An attempt has been made to investigate whether i.m. injection of P-32 in a single amount of 333 MBq/kg of body weight have any influence on the activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), alpha-hydroxy-butyrate dehydrogenase (HBDH), leucine aminopeptidase (LAP), gamma-glutamyl transpeptidase (GGT), alkaline phosphatase (AP) and cholinesterase (ChE), as well as on the concentration of total cholesterol (TC) total lipids (TL) calcium (Ca), inorganic phosphorus (P), and magnesium (Mg) in the blod plasma of chickens. The influence of P-32 upon red blood cell count, thrombocyte (T) and total leukocyte count and different leukocyte counts, hemoglobin concentration and hematocrit in chicken blood was also investigated. The activity of most enzymes was increased during the first five days and it was decreased on the 7th and 10th day of the experiment. The ChE did not change, and LAP and the concentration of TL was lower during the entire experimental period. The Ca concentration was decreased on the 1st and the 3rd day, the Mg concentratium was reduced on the 1st and the 7th day, and the P concentration was reduced on the 7th day after injection of P-32. The leucopenia and lymphocytopenia was noticed at the 1st day after injection of P-32. The other blood parameters were lower from the 5th day of experiment. Clinical signs did not appear before the 6th days after P-32 injection and two chickens died on the 9th and another two chickens died on the 10th day of the experiment. Pathomorfological and histological investigation of caracasses and organs of died and one sacrificed birds showed changes which are also tipical for acute internal radioactive contamination. The obtained data suggest that the determination of the enzyme activity in the blood plasma can serve as another qualitative test for diagnosis of damage caused by ionizing radiation in the chickens.

Keywords: radioactive phosphorus P-32, aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, alpha-hydroxy-butyrate dehydrogenase, leucine aminopeptidase, gamma-glutamyl transpeptidase, alkaline phosphatase, cholinesterase, total cholesterol, total lipids, calcium, phosphorus, magnesium, erytrocites, thrombocytes, leukocytes, lymphocytes, leukocytes differential count, hemoglobin, hematocrit, blood plasma, blood, clinical effects, pathological effect, chickens.

Research goals: As in our previous investigations - made upon the chickens of heavy breed (the experiments were completed until 1990) - we did not find any clinical or patomorphological changes on the chickens organs after the application of P-32 in amounts of 166,5 MBq/kg of body weight (according to the literature data, this dose is considered to be a half-lethal for some breeds), we administered a double dose of P-32 (333 MBq/kg of body weight) with the aim to determine: 1) whether a double dose of P-32 caused any changes on the chickens organs; 2) wheather, when and how eventual changes that can appear in the chicken organs, coused by ionizing radiation, can result in changes of biochemical parameters in the blood plasma (while the determination of hematologic parameters ought serve us as referent values), and 3) whether eventual changes of biochemical parameters in blood plasma of contaminated birds could serve for early detection of pathological and functional disorders of chickens organs, specially of those organs which are target for a certain radionuclide. As our performed investigation are fundamental (but also aimed), one can expect that obtained results can extended our knowledge about the effects of ionizing radiation upon the chickens of heavy breed in the case of their internal radioactive contamination. Besides, we expected that obtained results, or at least some of them, can have a practical value i.e. that they can help us in help us in early diagnozing of acute radioactive contamination. So far, our investigation have justified our expections.

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Last update: 10/24/95