Type of research: basic Duration from: 01/01/91. to 12/15/97. Papers on project (total): 19
Institution name: Veterinarski fakultet, Zagreb (53) Department/Institute: Department for Physiology and Radiobiology Address: Heinzelova 55 City: 10000 - Zagreb, Croatia
Communication
Phone: 385 (0)41 290 178
Summary: An attempt has been made to investigate whether i.m.
injection of P-32 in a single amount of 333 MBq/kg of body weight have
any influence on the activity of aspartate aminotransferase (AST),
alanine aminotransferase (ALT), lactate dehydrogenase (LDH),
alpha-hydroxy-butyrate dehydrogenase (HBDH), leucine aminopeptidase
(LAP), gamma-glutamyl transpeptidase (GGT), alkaline phosphatase (AP) and
cholinesterase (ChE), as well as on the concentration of total
cholesterol (TC) total lipids (TL) calcium (Ca), inorganic phosphorus
(P), and magnesium (Mg) in the blod plasma of chickens. The influence of
P-32 upon red blood cell count, thrombocyte (T) and total leukocyte count
and different leukocyte counts, hemoglobin concentration and
hematocrit in chicken blood was also investigated. The activity of most
enzymes was increased during the first five days and it was decreased on
the 7th and 10th day of the experiment. The ChE did not change, and LAP
and the concentration of TL was lower during the entire experimental
period. The Ca concentration was decreased on the 1st and the 3rd day,
the Mg concentratium was reduced on the 1st and the 7th day, and the P
concentration was reduced on the 7th day after injection of P-32. The
leucopenia and lymphocytopenia was noticed at the 1st day after injection
of P-32. The other blood parameters were lower from the 5th day of
experiment. Clinical signs did not appear before the 6th days after P-32
injection and two chickens died on the 9th and another two chickens died
on the 10th day of the experiment. Pathomorfological and histological
investigation of caracasses and organs of died and one sacrificed birds
showed changes which are also tipical for acute internal radioactive
contamination. The obtained data suggest that the determination of the
enzyme activity in the blood plasma can serve as another qualitative
test for diagnosis of damage caused by ionizing radiation in the
chickens.
Research goals: As in our previous investigations - made upon the
chickens of heavy breed (the experiments were completed until 1990) - we
did not find any clinical or patomorphological changes on the chickens
organs after the application of P-32 in amounts of 166,5 MBq/kg of body
weight (according to the literature data, this dose is considered to be a
half-lethal for some breeds), we administered a double dose of P-32 (333
MBq/kg of body weight) with the aim to determine: 1) whether a double dose
of P-32 caused any changes on the chickens organs; 2) wheather, when and
how eventual changes that can appear in the chicken organs, coused by
ionizing radiation, can result in changes of biochemical parameters in the
blood plasma (while the determination of hematologic parameters ought serve
us as referent values), and 3) whether eventual changes of biochemical
parameters in blood plasma of contaminated birds could serve for early
detection of pathological and functional disorders of chickens organs,
specially of those organs which are target for a certain radionuclide. As
our performed investigation are fundamental (but also aimed), one can
expect that obtained results can extended our knowledge about the effects
of ionizing radiation upon the chickens of heavy breed in the case of their
internal radioactive contamination. Besides, we expected that obtained
results, or at least some of them, can have a practical value i.e. that
they can help us in help us in early diagnozing of acute radioactive
contamination. So far, our investigation have justified our expections. Other information about the project.