SVIBOR - Project code: 3-03-303

MINISTRY OF SCIENCE AND TECHNOLOGY

Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69
E-mail: ured@znanost.hr

SVIBOR

SVIBOR - Collecting Data on Projects in Croatia

Project code: 3-03-303


FETURES OF GLUCONEOGENESIS IN METABOLIC DISORDERS OF DAIRY COWS


Main researcher: KAMPL, BRANIMIR (124766)


Assistants
Type of research: applied
Duration from: 01/01/91. to 12/31/95.
Papers on project (total): 36
Institution name: Veterinarski fakultet, Zagreb (53)
Department/Institute: Department of Patophysiology Veterinary Faculty University of Zagreb
Address: Heinzelova 55
City: 10000 - Zagreb, Croatia
Communication
Phone: 385 (0)1 2390-182
Fax: 385 (0)1 2390 184
Fax: 385 (0)1 214-697
Phone: 385 (0)01 2390 182
E-mail: kamplQmavef.vef.hr
Summary: In lactating dairy cows experiencing the metabolical disorders and normal cows with good productive and reproductive features, respectively, in the biopsates of liver tissue the activity of cytosolic NAD-malate dehydrogenase and cytosolic phosphoenolpyruvate carboxykinase are determinated in vivo. In addition, in the blood of experimental animals the contet of total lipids, total and free cholesterol, glucosis, free fatty acids, inorganic phosphorus and calcium are determinated. It is expected that the obtained results will provide more detailed insight into the gluconeogenic process in ruminants and give additional explanations on the patogenesis of metabolic disorders. In the course of the experiment, the new analytical methods giving insight into the metabolic status of the animals are applied, which are at the same time more practically applicable, namely the determination of urea and proteins in the milk.

Keywords: Dairy cows, liver, gluconeogenesis, blood serum, metabolic disorders.

Research goals: The objective of the proposed investigation is to examine the activity of extramitohondrial NAD-malat dehydrogenase (MD) and phosphoenolpyruvate carboxikynase (PEPCK) in the hepatocytes of high yielding cows in vivo, experiencing the metabolical disturbances (ketosis or ruminal acidosis - conditions which include metabolical acidosis), and in the normal cows with convenient productive and reproductive characteristics respectively. The degree of metabolical disturbances will be estimated by determinating the blood total lipids, total and free cholesterol, glucose, free fatty acids, ketone bodies and calcium and inorganic phosphorus. Since this field of ruminant phisiologyhas nor been yet investigated in detail, the results of it would supplement existing knoveledge on the activity of mentioned enzymes in the ruminants. In addition, it is expected that the obtained results would faciliate the insight into the extent of oxaloacetate formation from propionate in vivo in the animals with disturbances, and on the importance of this part of gluconeogenic pathway in the course of pathogenesis of metabolical disturbances. It is also expected that the results of the enzyme activity in liver could corelate with results of the measured parameters in blood and milk, what could be applicated in the diagnostics as an index of the development's degree of the metabolical disturbance. Finnaly, in the course of the work, the new methodology of enzyme and metabolite determinations will be gradually introduced.

COOPERATION - PROJECTS


  1. Name of project: 3-03-303 Biochemische und molekulaere Untersuchungen der physiologischen Mechanismen die die metabolische Funktion der Milchkuehen bestimmen
    Name of institution: Veterinaermedizinische Fakultaet der Ludwig-Maximilians Universitaet Muenchen
    City: Muenchen, Njemačka

COOPERATION - INSTITUTIONS


  1. Name of institution: Pik_ČAZMA, FARMA MLIJEČNIH KRAVA, GRABOVNICA
    Type of institution: State institute
    City: Budimpešta, Mađarska

  2. Name of institution: Farma mliječnih krava "Grabovnica" PIK Čazma
    Type of institution: State institute
    City: 43240 - Čazma, Hrvatska
Other information about the project.
MZT Croatian language SVIBOR Alphabetic list Sorted on project code Sorted on institutions Search help
Ministry of
Science and
Technology		 Croatian
language		 Svibor
homepage		 Alphabetic
list		 Sorted on
project code		 Sorted on
institutions		 Search Help
Last update: 10/14/95
Information: svibor@znanost.hr