SVIBOR - Project code: 3-03-480


Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69


SVIBOR - Collecting Data on Projects in Croatia

Project code: 3-03-480


Main researcher: MAKEK, ZDENKO (27491)

Type of research: basic
Duration from: 07/08/92. to 07/08/95.

Papers on project (total): 11
Institution name: Veterinarski fakultet, Zagreb (53)
Department/Institute: Department of Reproduction and Ostetrics
Address: Heinzelova 55
City: 10000 - Zagreb, Croatia
Phone: 385 (0) 2390-111
Phone: 385 (0) 2390-326
Fax: 385 (0) 21-46-97

Summary: MOET investigations (multiple ovulation end embriotransfer) were performed in more then 60 cows and heifers. Induction of superovulation was investigated using different hormone preparations (Folligon, Pregmanon, Folltropin, F.S.H.-P), sinchronization of estrus cycle (Anifertil), and the results of induced superovulation were followed up by ginecological (rectal and vaginal) and ultrasound examination. A 5 MHz transducer and a high-quality scanner were used for transrectal evaluation of the reproductive organs, primarly ovaries during a superstimulation regimens and superovulatory response (corpora lutea, follicles, cystic ovarian conditions). That way we could determine the number of corpora lutea and predict the recovery rates of embryos. Embryos were collected using non-surgical flushing (gravitation and aspiration) with PBS (Dulbeccos phosphate buffered saline) medium, and then evaluated and classified using IETS (International Embryo Transfer Society) criteria. One part of embryos were immediately transferred in sinchronized recipients and rest were cryopresevated using special mediums (glycerin) and plunged into liquid nitrogen (-196 degrees C). When we succeded to get new recipients, embryos were thawed by "one step" procedure, evaluated in accordance to IETS and transferred.

Keywords: Cow, heifer, donor, recipient, superovulation, echosonography, embryo, embryotransfer.

Research goals: The goals of this project in the first year were: investigationson on the possibilities for inducing superovulation, selection of the most reliable procedure, inspection and estimation of superovulatory responces by ultrasonography and, finally, flushing and transfer of collected embryos in recipients. Mentioned aims of this project for the first year were completely achieved, and besides we accomplished cryopreservation and thawing, and finally transfer of those embryos in three recipients. Further researches will be directed on the collection of the embryos from at least 50 donor cows, and transfer to at least 25 to 30 recipients. The collected embryos will be cryopreservated with a wiew to preserve and later use of possible overplus, untill the new recipients would be prepared. Besides, this procedure will be used to perform embryo transfer in as possible as more recipients, so in the beginning of the second year and during the third year of researche, we will have a specific comprehending in the efficiency of the cryopreservation/thawing procedures. This researche will be aimed on the improvement and simplification of the procedures used for freezing and thawing of bovine embryos. In that period our investigations will be directed on evaluation and categorization of collected embryos in accordance with international standards, and on the possibilities in improving the mediums for flushing, maturation, freezing and thawing of bovine embryos. Our investigations will be completed with performing a selection of the top cows of various breeds (40 to 50 cows), to form nucleus herds for quality embryos supply. In the contining of the project, investigations will be directed to maturation and fertilization of bovine oocytes in vitro.


  1. Name of project: 3-03-419 Ultrazvučna ehosonografija u veterinarskoj ginekologiji i akušerstvu
    Name of institution: Veterinarski fakultet Sveučilišta u Zagrebu
    City: 10000 - Zagreb, Croatia


  1. Name of institution: Farma mliječnih krava "Veliki Zdenci"
    Type of institution: Economical/Production
    Type of cooperation: Joint publishing of scientific papers
    City: Veliki Zdenci, HR

  2. Name of institution: Farma tovnih goveda "Poljanski Lug" Vrbovec
    Type of institution: Economical/Production
    Type of cooperation: Occasional exchange of information
    City: Vrbovec, HR

  3. Name of institution: Centar za reprodukciju u stočarstvu Hrvatske
    Type of institution: State institute
    Type of cooperation: Promotion or commercialization of results
    City: 10000 - Zagreb, Croatia

  4. Name of institution: Zavodi i klinike Veterinarskog fakulteta Zagreb
    Type of institution: University/Faculty
    Type of cooperation: Joint publishing of scientific papers
    City: 10000 - Zagreb, Croatia


  1. Name: Saznanja o postupcima embriotransfera u goveda prenosili smo i na veterinare na terenu, kao i na veterinare u farmama mliječnih krava, a novija saznanja i na polaznike našeg poslijediplomskog studija "Fiziologija i patologija reprodukcije goveda s U.O.".
    Type of achievement: Technology

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Last update: 10/06/95