SVIBOR - Project code: 4-01-047

MINISTRY OF SCIENCE AND TECHNOLOGY

Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69
E-mail: ured@znanost.hr

SVIBOR - Collecting Data on Projects in Croatia

Project code: 4-01-047

SYMBIOTIC EFFECTIVENESS OF AUTOCHTHONOUS Bradyrhizobium japonicum STRAINS

Main researcher: REDŽEPOVIĆ, SULEJMAN (40561)

Assistants

SIKORA, SANJA (85451)
MANITAŠEVIĆ, JELENA (71326)
ŠOŠKIĆ, MILAN (66031)
ŽAFRAN, JELENA (900443)

Type of research: applied
Duration from: 01/01/91. to 12/31/00.

Papers on project (total): 26

Institution name: Agronomski fakultet, Zagreb (178)
Department/Institute: DEPARTMENT OF MICROBIOLOGY
Address: Svetošimunska 25

Communication: Phone: 385 (0) 1 2335 777; Fax: 385 (0) 1 2335 777

Summary: Symbiotic nitrogen fixation has already given a significant ecological and economic contribution to agricultural production. Relating to the main research objective of this Project, detailed laboratory investigations, pot experiments under controlled conditions and field trials will be carried out to enable selection of the most effective symbiotic association of soybean genotypes and Bradyrhizobia strains. Investigations will involve isolation of autochthonous strains of B. japonicum; RAPD analysis (Random Amplified Polymorphic DNA), serological and antibiotic identification of strains; assessment of cultivar-strain compatibility; determination of nitrogenase and hydrogenase activity. Thus tested and thoroughly checked strains will be studied in field trials on different locations, with special attention being paid to the influence of mineral nitrogen fertilization upon the effectiveness of the B. japonicum strains selected. Isolated autochthonous strains, if any, possessing also the enzyme hydrogenase (hydrogen uptake positive), which are rare in nature, will be investigated in great detail. Our own collection of B. japonicum strains will be formed so as to preserve the original genetic material, which could be a prerequirement for connection to the developed countries.

Keywords: nitrogen fixation, Bradyrhizobium japonicum, nodule isolates, strain identification, RAPD analysis, serogroup, soybean (Glycine maw L.), symbiotic effectiveness, nitrogenase, hydrogenase

Research goals: The main goal is isolation and determination of autochthonous strains of Bradyrhizobium japonicum from different ecological regions of the Republic of Croatia to enable selection of the most efficient strains and determine the most effective symbiotic associations for the soybean genotype. Identification and selection of B. japonicum strains are important for the use of strains in preparing commercial inoculants for bacterization of soybean, as well as for preserving our own original genetic material for future research. There are reasons to believe that the quality of soybean, as a significant legume, can be improved by application of symbiotically bound nitrogen. Considerable economic effects can be achieved by the application of isolated, autochthonous, effective B. japonicum strains in the pre-seeding bacterization of soybean since the expensive mineral nitrogen can be reduced to a minimum. At the same time, ecological hazards related to excessive use of mineral nitrogen fertilizers are decreased. Investigations could also explain the existing interdependence between particular properties to be studied in the symbiotic system of the soybean genotype and B. japonicum strain, which could theoretically result in creation of more reliable criteria that would shorten selection paths of both partners.

Other information about the project.


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Last update: 10/09/95
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