SVIBOR - Project code: 1-07-184

MINISTRY OF SCIENCE AND TECHNOLOGY

Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69
E-mail: ured@znanost.hr

SVIBOR

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Project code: 1-07-184


Development of Potentiometric Sensors and Biosensors


Main researcher: SAK-BOSNAR, MILAN (130381)


Assistants
Type of research: basic
Duration from: 01/01/91. to 12/31/94.
Papers on project (total): 16
Papers on project quoted in Current Contents: 2
Institution name: Pedagoški fakultet, Osijek (122)
Department/Institute: Department of chemistry
Address: L. Jagera 9
City: 31000 - Osijek, Croatia
Communication
Phone: 385 (0)31 125 199
Fax: 385 (0)31 126 757
Summary: The goal of the investigations proposed, is the development of the following new types of potentiometric sensors (ion-selective electrodes) and biosensors: surfactant, calcium, magnesium, halogenide, enzyme sensor. The investigations should comprise the synthesis of sensor material, selection of sensor type and conducting substrate, selection of plasticizer and solvent mediator, response of the sensor towards the species investigated, analytical and physicochemical sensor characterization, application of the sensors for the quantification of species investigated, using direct potentiometry and potentiometric titration method. Biosensors (enzyme electrodes) should be characterized according to the following aspects: construction of enzyme electrode, measuring of actual enzyme activity, influence of the pH value and ionic strenght of the medium on enzyme activity, analytical characterization, the application possibilities for the following of enzyme reaction kinetic, investigation of the influence of inhibitors and activators etc.

Keywords: potentiometric sensor, ion-selective electrode, biosensor, enzyme electrode, surfactants, calcium, magnesium, enzymes, potentiometry, potentiometric titration,

Research goals: The main goals of proposed investigations are: -development of the rapid, simple, accurate and inexpensive analytical methods for routine determination of species investigated, in pure and complex materials -the overcoming of the disadvantages of the existing routine analytical methods (visual titrimetric methods, spectrophotometric methods etc.) for actual determination: visual detection of the end-point, time-consuming and labour intensive methods, interferences at the end-point location by the coloured and turbid samples, use of toxic organic solvents, etc. -the overcoming of the limitations of up to now developed ion-selective electrodes and biosensors: lack of selectivity and sensitivity, problems connected with conditioning of electrodes,poor mechanical properties by some types of electrodes, increasing of life-time of electrodes, etc. -reducing the analyte amount. The results of investigations should contribute to: -better understanding of the phenomenon on the ion/organic molecul-sensor interface, -better understanding of the reaction-mechanism inside the sensor's (ion-selective electrode, biosensor) membrane, -analysis (determination, monitoring) of specific chemical and biological/biochemical species, -introduction of new chemical/instrumental analysis of materials, -development and commercialization of chemical sensors and biosensors.

COOPERATION - INSTITUTIONS


  1. Name of institution: Ecochem
    Type of institution: Economical/Production
    Type of cooperation: Joint publishing of scientific papers
    City: 31000 - Osijek, Croatia

  2. Name of institution: Denit AG Chemicals
    Type of institution: Economical/Production
    Type of cooperation: Systematic exchange of information
    City: Zurich, Švicarska

  3. Name of institution: Metaport
    Type of institution: Economical/Production
    Type of cooperation: Joint publishing of scientific papers
    City: 10000 - Zagreb, Croatia

  4. Name of institution: Institut za medicinska istraživanja i medicinu rada
    Type of institution: State institute
    Type of cooperation: Systematic exchange of information
    City: 10000 - Zagreb, Croatia

  5. Name of institution: Farmaceutsko-biokemijski fakultet
    Type of institution: University/Faculty
    Type of cooperation: Occasional exchange of information
    City: 10000 - Zagreb, Croatia

  6. Name of institution: Prehrambeno-tehnološki fakultet
    Type of institution: University/Faculty
    Type of cooperation: Joint publishing of scientific papers
    City: 31000 - Osijek, Croatia
Other information about the project.
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Last update: 10/09/95
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