SVIBOR - Project code: 3-03-302

MINISTRY OF SCIENCE AND TECHNOLOGY

Strossmayerov trg 4, HR - 10000 ZAGREB
tel.: +385 1 459 44 44, fax: +385 1 459 44 69
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Project code: 3-03-302


CARBOHYDRATE METABOLISM IN DIABETES AND SIDEROPENIC ANEMIA


Main researcher: SVETINA, ANTE (45645)


Assistants
Type of research: applied
Duration from: 01/01/91. to 12/31/95.
Papers on project (total): 23
Papers on project quoted in Current Contents: 1
Institution name: Veterinarski fakultet, Zagreb (53)
Department/Institute: Department of Pathological Physiology
Address: Heinzelova 55
City: 10000 - Zagreb, Croatia
Communication
Phone: 385 (0)041 290-181
Fax: 385 (0) 041 214-697
Summary: The effect of endocrine pancreas cells transplantation on the activities of the key enzymes in glycolytic, gluconeogenic and pentose phosphate pathway in the liver of alloxan diabetic rats and mice are investigates, especially with regard to the duration of disease.The results are compared with the effect of insulin administration following several week. In the both groups of diabetic animals (that received transplantat or treated with insulin) the hepatic enzyme activities are compared with activities in healthy animals (healthy control) and in diabetic animals immediatly before the therapy is started (pre-therapy diabetic control), as well as in non-treated diabetic animals with the same duration of disease as therapied animals (parallel diabetic control). In the second part of our work we have investigated whether iron deficiency in postnatal anemia of pigs is associated with the changes in glucose metabolism. Namely, low transplacental exchange of iron, rapid neonatal growth, and diet inadequacy cause iron deficiency in baby pigs, which is economically impotrant disease in large pig production units. We have examined parallely hematological parameters, serum iron level, and the concentration of glucose and its precursors in the blood. In addition, the activity of some regulatory enzymes in hepatic glucose metabolism are measured. The results are compared with the results obtined on healthy pigs (iron supplemented group).

Keywords: alloxan diabetes,rats,mice,insulin,endocrine pancreas cells,transplantation,pyruvate kinase,fructose-1,6-bisphosphatase,glucose-6-phosphatase,citrate cleavage enzyme,anemia,pig,iron deficiency,erythrocyte parameters,glucose,lactate,nonesterified fatty acids.

Research goals: The main goal the first part of investigation is to establish in which extend, and how long, hepatic carbohydrate and lipid metabolism is corrected after endocrine pancreas cells transplantation in comparison with insulin therapy. Additionally,we can get an information about the time in which the transplantation give the best results. In the second part of investigations, the parallel determinations of hematological parameters with the concentration of glucose and some regulatory gluconeogenic substrates (lactate,pyruvate) in the blood, and also with hepatic enzyme activites, could demonstrate whether increased glucose dependance exist in iron-deficient postnatal anemia of pigs. Additionaly, the results can contribute to our understanding whether hypoglicaemia of neonatal pigs is associated with increased glucose metabolism induced by iron deficiency.

COOPERATION - INSTITUTIONS


  1. Name of institution: Institut "Ruđer Bošković"
    Type of institution: University/Faculty
    City: 10000 - Zagreb, Croatia

  2. Name of institution: Svinjogojska farma "Gradac", PIK Vrbovec
    Type of institution: State institute
    City: 43216 - Vrbovec, Hrvatska
Other information about the project.
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Last update: 10/04/95
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