EFFECTS OF PESTICIDES AND MYCOTOXINES ON SWINE REPRODUCTION
Main researcher
: GOJMERAC, TIHOMIRA (98185) Assistants
ŽURIĆ, MARIJAN (118411)
TOPOLKO, STJEPAN (50292)
MANDIĆ, BOŽICA (98400)
AUSLENDER-UJEVIĆ, VERA (151336)
MITAK, MARIO (161734)
BILANDŽIĆ, NINA (900695)
Type of research: applied Duration from: 04/30/91. to 12/30/93. Papers on project (total): 14
Papers on project quoted in Current Contents: 3
Institution name: Veterinarski institut, Zagreb (48) Department/Institute: Department of residues analysis Address: Savska 143 City: 10000 - Zagreb, Croatia
Communication
Phone: 385 (0)41 53 50 11
Fax: 385 (0)41 53 71 40
Summary: The study of the effect of atrazine
(2-chloro-4-ethylamino-6-isopropylamino-s triazine),an s-triazine
pesticide (herbicide),and zearalenone,a mycotoxin, on the ovarian function
of pigs (gilts) in intensive breeding,included the following:a/
determination of atrazine residues in pig drinking water on a pig-breeding
farm,using the immunoenzymatic method (ELISA) compared to high performance
liquid chromatography (HPLC). b/ determination of zearalenone residues in
corn kernels as the main ingredient of pig feed,using the immunoenzymatic
method (ELISA).c/determination of zearalenone concentration in serum of
pigs with reproduction disturbances, using the immunoenzymatic method and
d/ follow-up of the effect of subtoxic doses of atrazine on the ovarian
function,i.e. occurrence of estrus and ovulation in gilts.Serum
concentration of 17á-estradiol (17á-E) and progesterone (P), the endocrine
secretion products that play an important role in the cyclic ovarian
activity,were determined in gilts administered subtoxic doses of atrazine
in feed during 19 days of estrouscycle.Determination of 17á-E and P in
serum was performed on the days of the expected proestrus,estrus and
metestrus.The animals in which the signs of the next estrus failed to
occur,were sacrificed 8 days later. The ovary and uterus tissues were
removed and histopathologic examinations were performed.During the
study,histopathologic analysis revealed alterations in the liver tissue
obtained from the atrazine-treated pigs.Therefore,the study was extended
to the follow-up of hepato- toxic effect of the contaminant in pigs,which
included determination of the avtivities of alanine aminotransferase
(ALT),aspartate aminotransferase (AST),gamma-glutamyltransferase (GGT) and
alkaline phosphatase (AP).
Research goals: Disturbances of the reproduction cycle in pigs cause
considerable economic damage in intensive pig breeding.These disturbances
manifest as frequent estrus failure or poor expression of the estrus
signs.These reproduction disturbances of non-infective etiology may be
caused by toxic substances,e.g.,atrazine,a pesticide (herbicide) and
zearalenone,a mycotoxin,that reach the animal body through feed and water.
Results of the study clearly pointed to the toxic effect of these
contaminants on the ovarian function in gilts.As subtoxic amounts of
atrazine and zearalenone can be concurrently present in the pig feed,their
individual effects and possible interaction of equimolar amounts of these
substances on the ovarian function in pigs in the farm-breeding conditions
were also investigated. Comparison of biochemical and histopathologic
examination has suggested that results of the study of individual effects
of these contaminants and their equimolar mixtures on ovarian function in
pigs might be expected to contribute to the clarification of some estrous
questions in the pathology of pig reproduction,such as failure,delay or
poor expression of estrus.This should be preceded by monitoring of
atrazine residues in pig drinking water and determination of zearalenone
residues in corn and pig serum. Other information about the project.